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Ovcar3 mutation

WebHTB-161 ™. NIH:OVCAR-3 [OVCAR3] are epithelial cells that were isolated in 1982 from the malignant ascites of a patient with progressive adenocarcinoma of the ovary. Use these cells to study drug resistance in ovarian cancer. Product category. WebTo cite this cell line use: OVCAR-3 (RRID:CVCL_0465) Comments Part of: AKT genetic alteration cell panel (ATCC TCP-1029). Part of: Cancer Dependency Map project …

BRCA1/2 mutation analysis in 41 ovarian cell lines reveals

WebIt describes the source of the mutation i.e gene name/sample name/tissue name with unique ID, and also shows the mutation syntax at the amino acid and nucleotide sequence level. You can see more information on our help pages . Genomic Mutation ID COSV52661282 Legacy Identifier COSM10758 Gene name TP53 AA mutation WebJul 24, 2024 · Basal phosphorylated (pCHK1) and total CHK1 levels were also especially increased in CCNE1AMP lines (OVCAR3, FUOV1, and COV318), consistent with high levels of replication stress 49, 50 in these... lehigh university phd english https://be-night.com

High-grade serous ovarian cancer cell lines exhibit …

WebThe Caov-3 cells harbor a nonsense mutation in the p53 gene, and have multiple copies of the ovarian cancer oncogene PIK3CA. They are sensitive to vinblastine, cisplatin, and … WebJul 1, 2024 · We found that, with the exception of OVCAR3, HGSC cell lines COV318, COV362, KURAMOCHI, OVCAR4, and OVSAHO, generally demonstrate poor growth as either subcutaneous or intraperitoneal xenografts.... WebJun 30, 2011 · Molecular analysis of the WNT4 gene permitted us to identify a new mutation (p.A233T). Functional studies revealed partial repression of steroidogenic enzymes (normal repression of HSD3B2) contrasting with the abnormal reexpression of CYP17A1 enzyme in the OVCAR3 cell line. lehigh university parents weekend 2022

NIH:OVCAR-3 [OVCAR3] - HTB-161 ATCC

Category:In vivo tumor growth of high-grade serous ovarian cancer cell lines

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Ovcar3 mutation

Mechanisms underlying p53 regulation of PIK3CA transcription in …

WebApr 30, 2024 · Ovcar3 cells contain a R248Q mutation , which is a dominant negative mutation in the DNA binding domain of p53 . Kuramochi cells, considered to have one of the most closely matching genetic profiles to primary patient tumors of all commercially available ovarian cancer cell lines [ 25 ], contain a dominant negative D281Y mutation … WebBradbury Characterisation Cancers - HTG Molecular

Ovcar3 mutation

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WebMaterials and methods: We investigated the anticancer effects of combination treatment with prexasertib (LY2606368), a selective ATP competitive small molecule inhibitor of Chk1 and Chk2, and rucaparib, a PARP inhibitor, in BRCA wild-type ovarian cancer cell lines (OVCAR3 and SKOV3). http://igrcid.ibms.sinica.edu.tw/cgi-bin/cell_line_view.cgi?cl_name=OVCAR3

WebIn a separate in vitro study, the proliferation of 26 of 39 representative human ovarian cancer cell lines bearing a wide range of HR deficiencies other than a BRCA mutation was inhibited by 50% ...

WebPanel A shows the results of Western blot analysis of the cell-culture medium or cell extracts of ovarian adenocarcinoma cells (OVCAR3) after they were transfected with mutant WNT4, wild-type... WebEffect of FAK, DLC-1 gene expression on OVCAR-3 proliferation was examined by FCM and Cell Counting Kit-8 (CCK-8) methods. Results showed that DLC-1 gene high expression …

WebA cell line, NIH:OVCAR-3, has been established from the malignant ascites of a patient with progressive adenocarcinoma of the ovary after combination chemotherapy with …

WebNIH:OVCAR-3 [OVCAR3] are epithelial cells that were isolated in 1982 from the malignant ascites of a patient with progressive adenocarcinoma of the ovary. Use these cells to … lehigh university physics departmentWebJan 1, 2024 · CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats) mediated genome editing is a powerful approach for loss of function studies. Here we report that lentiviral CRISPR/Cas9 vectors are highly efficient in introducing mutations in the precursor miRNA sequence, thus leading to the l … lehigh university pay billWebNational Center for Biotechnology Information lehigh university-philip rauch fieldhouseWebJun 6, 2024 · Next, we explored the functional role of miR-223-3p in ovarian cancer using SKOV3 and OVCAR3 cell lines. Our results suggested that miR-223-3p mimic promoted ovarian cancer cell proliferation, migration, and invasion in vitro. ... GAPDH was used as loading control. wt: wild type; mut: mutation; * p < 0.05. Figure 5. Overexpression of … lehigh university phd programsWebDescription The Caov-3 cell line is a primary ovarian cancer cell line with epithelial morphology. These cells form tightly packed colonies in adherent culture. All-trans retinoic acid has been shown to suppress the growth of Caov-3 ovarian carcinoma cells in vitro. These cells express the NB/70K, CA-125, Ba-2, and Ca-1 tumor-associated antigens. … lehigh university phd psychologyWebOVCAR3 cells represented high grade serous ovarian cancers with the oncogenic mutation R248Q. As shown in Figure 1A, ... Mutations in p53 are of various types and can be broadly categorized as either loss of function (p53 null, as seen in Hec50 cells) or gain of function, which are oncogenic mutations caused by single amino acid alternations ... lehigh university philharmonicWebJul 17, 2024 · Characterisation of Ovarian Cancer Cell Line NIH-OVCAR3 and Implications of Genomic, Transcriptomic, Proteomic and Functional DNA Damage Response … lehigh university physics